Anti-ENAH/MENA antibody [EPR5660] (ab124685)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5660] to ENAH/MENA
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ENAH/MENA antibody [EPR5660]
See all ENAH/MENA primary antibodies -
Description
Rabbit monoclonal [EPR5660] to ENAH/MENA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
corresponding to Human ENAH/MENA aa 550-650.
Database link: Q8N8S7 -
Positive control
- MDA-MB-435, MCF7, HUVEC and A431 cell lysates; Human Uterus tissue.
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General notes
This product was previously labelled as ENAH
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5660 -
Isotype
IgG -
Research areas
Images
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ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution
Lane 1 : MDA-MB-435 (Human ductal carcinoma cell line) cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
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