Anti-SOX3 antibody (ab183606)
Key features and details
- Rabbit polyclonal to SOX3
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SOX3 antibody
See all SOX3 primary antibodies -
Description
Rabbit polyclonal to SOX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human SOX3 aa 1-159. The exact sequence is proprietary.
Database link: P41225 -
Positive control
- WB: MCF7 whole cell lysate. IHC-P: Mouse and rat brain tissue ICC/IF: U-87 MG cells. IP: NT2D1
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (ab183606)
Immunohistochemical analysis of mouse brain tissue sections labeling SOX3 with ab183606 at 1/500. Sections were embedded in Paraffin.
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Western blot - Anti-SOX3 antibody (ab183606)Anti-SOX3 antibody (ab183606) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 45 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SOX3 antibody (ab183606)Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (human glioblastoma astrocytoma) cells labeling SOX3 with ab183606 at 1/500 (Green, nuclear expression). The cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Beta tubulin 3 was counterstained with mouse monoclonal anti-Beta Tubulin 3 antibody (red).
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Immunoprecipitation - Anti-SOX3 antibody (ab183606)ab183606 at 5µg immunoprecipitating SOX3 protein in NT2D1 (human embryonal carcinoma) whole cell lysate (lane 2).
Lane 1: NT2D1 whole cell lysate
Lane 2: ab183606 + NT2D1 whole cell lysate
Western blot was performed using ab183606 followed by anti-rabbit IgG as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (ab183606)Immunohistochemical analysis of mouse brain tissue sections labeling SOX3 with ab183606 at 1/500. Sections were embedded in Paraffin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (ab183606)Immunohistochemical analysis of rat brain tissue sections labeling SOX3 with ab183606 at 1/200. A goat anti-rabbit IgG was used as the secondary antibody. Sections were embedded in Paraffin.
