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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins

Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)

Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to E Cadherin (phospho S838 + S840)
  • Suitable for: WB, IP
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-E Cadherin (phospho S838 + S840) antibody
    See all E Cadherin primary antibodies
  • Description

    Rabbit polyclonal to E Cadherin (phospho S838 + S840)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human E Cadherin (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P12830

  • Positive control

    • WB: MCF7 whole cell extract; Rat brain tissue extract. IP: MCF7 whole cell extract.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Images

  • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
    Western blot - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
    Anti-E Cadherin (phospho S838 + S840) antibody (ab226779) at 1/10000 dilution + Rat brain tissue extract at 50 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
    Immunoprecipitation - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)

    E Cadherin (phospho S838 + S840) was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 µg ab226779. Western blot was performed from the immunoprecipitate using ab226779.

    Lane 1: MCF7 whole cell extract.

    Lane 2: Control IgG IP in MCF7 whole cell extract.

    Lane 3: ab226779 IP in MCF7 whole cell extract.

  • Western blot - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
    Western blot - Anti-E Cadherin (phospho S838 + S840) antibody (ab226779)
    All lanes : Anti-E Cadherin (phospho S838 + S840) antibody (ab226779) at 1/10000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) treated with CIP (calf-intestinal alkaline phosphatase), whole cell extract
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) treated with pervanadate, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 97 kDa



    5% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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