Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Monkey

Overview

  • Product name

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free
    See all Desmoglein 2/DSG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6768] to Desmoglein 2/DSG2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HepG2, HeLa, Human fetal heart, mouse heart and rat heart lysates. IHC-P: Human thyroid gland papillary carcinoma tissue.

  • General notes

    This product was previously labelled as Desmoglein 2.

    ab271913 is the carrier-free version of ab150372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
    Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
    All lanes : Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution

    Lane 1 : Trypsinised HAP1 cell lysate
    Lane 2 : Scraped HAP1 cell lysate
    Lane 3 : Trypsinised DSG2 knockout HAP1 cell lysate
    Lane 4 : Scraped DSG2 knockout HAP1 cell lysate
    Lane 5 : Trypsinised A431 cell lysate
    Lane 6 : Scraped A431 cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab150372).

    Lanes 1 - 6: Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150372).
  • Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)
    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free (ab271913)

© 2021 Astana Biomed Group LLP. All rights reserved.