Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)
![Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)](https://www.abcam.com/ps/products/269/ab269978/Images/ab269978-369991-ICCAP439715901.jpg "Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)")
Key features and details
- Mouse monoclonal [6D8] to Desmoglein 2/DSG2 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free
See all Desmoglein 2/DSG2 primary antibodies -
Description
Mouse monoclonal [6D8] to Desmoglein 2/DSG2 - BSA and Azide free -
Host species
Mouse -
Specificity
This antibody does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain of Desmoglein 2/DSG2. The clone number has been updated from (3G132) to (6D8) both clone numbers name the same antibody clone.
-
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: A431 cells. WB: HAP1 wild-type whole cell lysate.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab269978 is the carrier-free version of ab14415. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
6D8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab14415).
ab14415 staining Desmoglein 2 in A431 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab14415 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
![Western blot - Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)](https://www.abcam.com/ps/products/269/ab269978/Images/ab269978-370010-AntiDesmoglein-2-DSG2-6D8-BSA-Azide-free-western-blot-hap1-wild-type-human.jpg)
Western blot - Anti-Desmoglein 2/DSG2 antibody [6D8] - BSA and Azide free (ab269978)All lanes : Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415) at 3 µg/ml
Lane 1 : HAP1 wild-type whole cell lysate
Lane 2 : HAP1 DSG2 knockout whole cell lysate
Lane 3 : A431 wild-type whole cell
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab14415).
ab14415 was shown to specifically react with DSG2 (Desmoglein 2) in wild type HAP1 cells. No band was observed when DSG2 (Desmoglein 2) knockout samples were used. Wild-type and DSG2 (Desmoglein 2) knockout samples were subjected to SDS-PAGE. The membrane was blocked for an hour using 5% milk before ab14415 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 3ug/ml and a 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
