Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415)
![Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415)](https://www.abcam.com/ps/products/14/ab14415/Images/ab14415-21919-anti-desmoglein-2-antibody-6d8-immunohistochemistry.jpg "Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415)")
Key features and details
- Mouse monoclonal [6D8] to Desmoglein 2/DSG2
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Desmoglein 2/DSG2 antibody [6D8]
See all Desmoglein 2/DSG2 primary antibodies -
Description
Mouse monoclonal [6D8] to Desmoglein 2/DSG2 -
Host species
Mouse -
Specificity
This antibody does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain of Desmoglein 2/DSG2. The clone number has been updated from (3G132) to (6D8) both clone numbers name the same antibody clone.
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Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Desmoglein 2/DSG2.
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General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
This product was previously labelled as Desmoglein 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ascites -
Purification notes
Purified from ascites. -
Clonality
Monoclonal -
Clone number
6D8 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415) This image is courtesy of an Abreview submitted by Dr Rudolf Jungab14415 at a 1/10 dilution staining human urothelium (bladder cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes following heat mediated antigen retrieval and paraformaldehyde fixation.
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![Western blot - Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415)](https://www.abcam.com/ps/products/14/ab14415/Images/ab14415-21918-anti-desmoglein-2-antibody-6d8-western-blot.jpg)
Western blot - Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Desmoglein 2/DSG2 antibody [6D8] (ab14415) at 3 µg/ml
Lane 1 : U2OS (Hu) whole cell lysate
Lane 2 : 293T (Hu) whole cell lysate
Lane 3 : Cos-7 (AGMk) whole cell lysate
Lane 4 : CHO (Hm) whole cell lysate
Lane 5 : PC12 (Rat) whole cell lysate
Lane 6 : 3T3 (Ms) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThe blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.
The antibody detects a species of the predicted mobility (125 kDa) in Human cell lines U2OS (1) and 293T (2). No signal was observed in extracts from other species, suggesting that this antibody does not cross-react with AGMk, Hm, Ms or Rat.
