Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP378Y] to SHIP-1 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IHC-P, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free
    See all SHIP-1 primary antibodies

  • Description

    Rabbit monoclonal [EP378Y] to SHIP-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human spleen tissue, Daudi cell lysate.

  • General notes

    ab190551 is the carrier-free version of ab45142 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab190551 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    All lanes : Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/5000 dilution (purified)

    Lane 1 : Daudi cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/1000 dilution (purified) + KM3 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with purified ab45142 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunocytochemistry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunocytochemistry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used. Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
  • Immunocytochemistry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunocytochemistry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    This data was developed using ab45142, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling SHIP-1 with purified ab45142 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used. Control 1: primary antibody (1/150) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
  • Flow Cytometry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Flow Cytometry - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    This data was developed using ab45142, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of Raji cells labelling SHIP-1 with purified ab45142 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  • Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunoprecipitation - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

    ab45142 (purified) at 1/20 immunoprecipitating SHIP-1 in Daudi whole cell lysate.

    Lane 1 (input): Daudi whole cell lysate (10µg)

    Lane 2 (+): ab45142 + Daudi whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45142 in Daudi whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Western blot - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Anti-SHIP-1 antibody [EP378Y] (ab45142) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 133 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    This data was developed using ab45142, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    This data was developed using ab45142, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling SHIP-1 with unpurified ab45142 at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)
    Anti-SHIP-1 antibody [EP378Y] - BSA and Azide free (ab190551)

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