Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1938Y] to GSTK1
Suitable for: ICC, WB, IP, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-GSTK1 antibody [EP1938Y]
See all GSTK1 primary antibodies
Description

Rabbit monoclonal [EP1938Y] to GSTK1
Host species

Rabbit
Specificity

This antibody reacts with GSTK1.

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human GSTK1 aa 150-250 (C terminal). The exact sequence is proprietary.
Positive control
- WB: HEK293T, HeLa and Daudi cell lysates; Fetal liver lysate. IHC-P: Human liver tissue. ICC: A549 cells. IP: Human heart lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as Glutathione S Transferase kappa 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1938Y
Isotype

IgG
Research areas

Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)

All lanes : Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTK1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 25 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab52759 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.

ab52759 Anti-GSTK1 antibody [EP1938Y] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used. Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE. ab52759 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry - Anti-GSTK1 antibody [EP1938Y] (ab52759)

Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling GSTK1 with purified ab52759 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.13 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EP1938Y] (ab52759)

Immunohistochemical staining of paraffin-embedded human liver using ab52759 at a 1:100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] (ab52759)

Purified ab52759 at 1/50 dilution (2µg) immunoprecipitating GSTK1 in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab52759 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52759 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa
Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)

Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/5000 dilution + Fetal liver lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Anti-GSTK1 antibody [EP1938Y] (ab52759)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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