Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSTK1 antibody [EP1938Y] (ab52759)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1938Y] to GSTK1
  • Suitable for: ICC, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GSTK1 antibody [EP1938Y]
    See all GSTK1 primary antibodies

  • Description

    Rabbit monoclonal [EP1938Y] to GSTK1

  • Host species

    Rabbit

  • Specificity

    This antibody reacts with GSTK1.

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GSTK1 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, HeLa and Daudi cell lysates; Fetal liver lysate. IHC-P: Human liver tissue. ICC: A549 cells. IP: Human heart lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Glutathione S Transferase kappa 1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    All lanes : Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : GSTK1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 25 kDa
    Observed band size: 27 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab52759 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab52759 Anti-GSTK1 antibody [EP1938Y] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used.  Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE.  ab52759 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Immunocytochemistry - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling GSTK1 with purified ab52759 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.13 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EP1938Y] (ab52759)

    Immunohistochemical staining of paraffin-embedded human liver using ab52759 at a 1:100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Immunoprecipitation - Anti-GSTK1 antibody [EP1938Y] (ab52759)

    Purified ab52759 at 1/50 dilution (2µg) immunoprecipitating GSTK1 in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab52759 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52759 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 25 kDa

  • Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Western blot - Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Anti-GSTK1 antibody [EP1938Y] (ab52759) at 1/5000 dilution + Fetal liver lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Anti-GSTK1 antibody [EP1938Y] (ab52759)
    Anti-GSTK1 antibody [EP1938Y] (ab52759)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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