Anti-E Cadherin antibody [CDH1/1525] (ab219332)
![Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275748-anti-e-cadherin-antibody-cdh1-1525-western-blot.jpg "Anti-E Cadherin antibody [CDH1/1525] (ab219332)")
Key features and details
- Mouse monoclonal [CDH1/1525] to E Cadherin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-E Cadherin antibody [CDH1/1525]
See all E Cadherin primary antibodies -
Description
Mouse monoclonal [CDH1/1525] to E Cadherin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Does not react with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Human E Cadherin.
Database link: P12830 -
Positive control
- WB: Human stomach tissue lysate IHC-P: Human colon carcinoma, skin, prostate carcinoma and placenta tissues ICC: MCF7 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CDH1/1525 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-E Cadherin antibody [CDH1/1525] (ab219332)All lanes : Anti-E Cadherin antibody [CDH1/1525] (ab219332) at 1 µg/ml
Lane 1 : Recombinant E-Cadherin protein
Lane 2 : Human stomach lysate
Predicted band size: 97 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-8-anti-e-cadherin-antibody-immunohistochemistry-breast-adenocarcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hepatocellular Carcinoma tissue labelling E Cadherin with ab219332 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
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![Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-7-anti-e-cadherin-antibody-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling E Cadherin with ab219332 at 2 µg/mL (green). The nuclear counterstain is Reddot (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275737-anti-e-cadherin-antibody-cdh1-1525-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275738-anti-e-cadherin-antibody-cdh1-1525-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemical analysis of formalin fixed, paraffin embedded human skin tissue labeling E Cadherin with ab219332 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275739-anti-e-cadherin-antibody-cdh1-1525-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemical analysis of formalin fixed, paraffin embedded human prostate carcinoma tissue labeling E Cadherin with ab219332 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275740-anti-e-cadherin-antibody-cdh1-1525-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling E Cadherin with ab219332 at 2 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)](https://www.abcam.com/ps/products/219/ab219332/Images/ab219332-275741-anti-e-cadherin-antibody-cdh1-1525-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 µg/mL.
