Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18307] to CTNNA3 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Dog, Human

Overview

  • Product name

    Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free
    See all CTNNA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18307] to CTNNA3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250780 is the carrier-free version of ab184916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250780 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 3 : Rat1 (Rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa


    Exposure time: 3 minutes


    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa


    Exposure time: 30 seconds


    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    This data was developed using ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa


    Exposure time: 1 second


    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    This data was developed using ab184916, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Western blot - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 100 kDa
    Observed band size: 100 kDa


    Exposure time: 1 second


    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

    This data was developed using ab184916, the same antibody clone in a different buffer formulation.

    CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HEK-293 whole cell lysate, 10 µg (Input).

    Lane 2: ab184916 IP in HEK-293 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184916 in HEK-293 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)
    Anti-CTNNA3 antibody [EPR18307] - BSA and Azide free (ab250780)

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