Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17990-13] to TGFBI - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free
    See all TGFBI primary antibodies

  • Description

    Rabbit monoclonal [EPR17990-13] to TGFBI - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat eyeball and liver lysates; Mouse eyeball and spleen lysates.

  • General notes

    Ab228133 is the carrier-free version of ab187085. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228133 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)
    Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)

    TGFBI was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab187085 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187085 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse eyeball lysate 10 µg (Input). 

    Lane 2: ab187085 IP in mouse eyeball lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187085 in mouse eyeball lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187085).

  • Western blot - Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)
    Western blot - Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)
    All lanes : Anti-TGFBI antibody [EPR17990-13] (ab187085) at 1/1000 dilution

    Lane 1 : Rat eyeball lysate at 20 µg
    Lane 2 : Mouse eyeball lysate at 20 µg
    Lane 3 : Mouse spleen lysate at 10 µg
    Lane 4 : Rat liver lysate at 10 µg

    Secondary
    Lanes 1 & 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 75 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1: 3 minutes; Lane 2: 1 second; Lanes 3 and 4: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with what has been described in the literature (PMID: 19478074).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187085).

  • Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)
    Anti-TGFBI antibody [EPR17990-13] - BSA and Azide free (ab228133)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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