Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)
![Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)](https://www.abcam.com/ps/products/251/ab251487/Images/ab251487-406168-anti-hnrnp-a1-citrulline-r122-antibody-epr20177-bsa-and-azide-free-western-blot.jpg "Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20177] to hnRNP A1 (citrulline R122) - BSA and Azide free
- Suitable for: Dot blot, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20177] to hnRNP A1 (citrulline R122) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Recombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251487 is the carrier-free version of ab208029. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251487 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20177 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)All lanes : Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] (ab208029) at 1/2000 dilution
Lanes 1 & 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 8 secondsThis data was developed using ab208029, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)](https://www.abcam.com/ps/products/251/ab251487/Images/ab251487-2-anti-hnrnp-a1-citrulline-r122-antibody-epr20177-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)This data was developed using ab208029, the same antibody clone in a different buffer formulation.Dot blot analysis of hnRNP A1 (citrulline R122) labeled with ab208029 at 1/1000 dilution. Lane 1: hnRNP A1 (citrulline R122) peptide; Lane 2: hnRNP A1 non-citrulline peptide; Lane 3: hnRNP A2B1 (citrulline R129) peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes. Based on sequence homology this antibody cross reacts with hnRNP A2B1 (citrulline R129) and hnRNP A1L2 (citrulline R122). -
![Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)](https://www.abcam.com/ps/products/251/ab251487/Images/ab251487-1-anti-hnrnp-a1-citrulline-r122-antibody-epr20177-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)This data was developed using ab208029, the same antibody clone in a different buffer formulation.hnRNP A1 (citrulline R122) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours whole cell lysate with ab208029 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208029 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours whole cell lysate 10 µg (Input). Lane 2: ab208029 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208029 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds. -
![Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)](https://www.abcam.com/ps/products/251/ab251487/Images/ab251487-4-benefits-of-recombinant-antibodies.png)
Anti-hnRNP A1 (citrulline R122) antibody [EPR20177] - BSA and Azide free (ab251487)