Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 123 964 ₸

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Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
See all Fibrillarin primary antibodies
Description

Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Fibrillarin aa 250 to the C-terminus.
Database link: P22087
Positive control
- 293T, HeLa, HepG2, HT-29 and MOLT4 cell lysates; Human liver and Human testis tissues; HeLa cells. Permeabilized MOLT4 cells; Immunoprecipitation pellet from HepG2 whole cell lysate (ab7900).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10823(B)
Isotype

IgG
Research areas

Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MOLT4 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Fibrillarin (green) with purified ab166630 at 1/5000. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

ab166630 showing +ve staining in Human gastric adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

ab166630 showing +ve staining in Human normal tonsil.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

ab166630 showing +ve staining in Human melanoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

ab166630 showing +ve staining in Human glioma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Fibrillarin with ab166630 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Immunofluorescent analysis of HeLa cells labeling Fibrillarin with ab166630 at a 1/100 dilution.
Flow Cytometry - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Flow cytometric analysis of permeabilized MOLT4 cells using ab166630 at a 1/10 dilution (red) or a Rabbit IgG (negative) (green).
Western blot - Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate at 10 µg

Predicted band size: 33 kDa
Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)

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