Anti-Lefty antibody [7C5G1H6H10] (ab34593)
![Anti-Lefty antibody [7C5G1H6H10] (ab34593)](https://www.abcam.com/ps/products/34/ab34593/Images/ab34593wb.bmp "Anti-Lefty antibody [7C5G1H6H10] (ab34593)")
Key features and details
- Mouse monoclonal [7C5G1H6H10] to Lefty
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lefty antibody [7C5G1H6H10]
See all Lefty primary antibodies -
Description
Mouse monoclonal [7C5G1H6H10] to Lefty -
Host species
Mouse -
Specificity
This antibody reacts with a 30 kDa protein corresponding to human Lefty. No reactivity occurs against 6X HIS tag. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant 6X HIS tagged human Lefty.
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General notes
Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7C5G1H6H10 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Lefty antibody [7C5G1H6H10] (ab34593)All lanes : Anti-Lefty antibody [7C5G1H6H10] (ab34593) at 1/2000 dilution
Lane 1 : 1µg 6X His tagged unrelated protein (shows that the antibody does not recognize the epitope tag).
Lane 2 : 1µg partially purified recombinant 6X his tagged human Lefty
Secondary
All lanes : IRDyeTM800 Goat anti-mouse IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
