Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E304] to Cdk2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk2 antibody [E304] - BSA and Azide free
    See all CDK2 primary antibodies

  • Description

    Rabbit monoclonal [E304] to Cdk2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Epitope

    The epitope is within the C-terminus of human Cdk2

  • Positive control

    • HeLa cells HeLa whole cell lysate (ab150035).

  • General notes

    Ab208697 is the carrier-free version of ab32147. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab208697 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Western blot - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    This WB data was generated using the same anti-Cdk2 antibody clone, E304, in a different buffer formulation (cat# ab32147).

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)
    Lanes 1 and 2: Green signal from target – ab32147 observed at 34 kDa
    Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
    Lanes 5 and 6: Merged (red and green) signal

    ab32147 was shown to specifically react with CDK2 when CDK2 knockout samples were used. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab32147 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Flow Cytometry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Cdk2 with purified ab32147 at 1/80 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32147).

  • Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    Clone E304 (ab208697) has been successfully conjugated by Abcam. This image was generated using Anti-Cdk2 antibody [E304] (Alexa Fluor® 647). Please refer to ab206038 for protocol details.

    ab206038 staining Cdk2 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206038 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunoprecipitation - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunoprecipitation - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    ab32147 (purified) at 1/40 immunoprecipitating Cdk2 from HeLa cells(Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32147).

  • Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    ab32147 staining Cdk2 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). ab150078 (1/500) an Alexa Fluor® 555-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32147).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    ab32147 staining Cdk2 in human squamous cell carcinoma of cervix tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32147).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    ab32147 staining Cdk2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32147).

  • Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Immunocytochemistry - Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

    This ICC/IF data was generated using the same anti-Cdk2 antibody clone, E304, in a different buffer formulation (cat# ab32147).

    ab32147 staining Cdk2 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/200). ab150078 (1/500) an Alexa Fluor® 555-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)
    Anti-Cdk2 antibody [E304] - BSA and Azide free (ab208697)

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