Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2234Y] to CDK2 (phospho T14)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Chinese hamster

Overview

  • Product name

    Anti-CDK2 (phospho T14) antibody [EP2234Y]
    See all CDK2 primary antibodies

  • Description

    Rabbit monoclonal [EP2234Y] to CDK2 (phospho T14)

  • Host species

    Rabbit

  • Specificity

    This antibody detects Cdk2 phosphorylated on Threonine 14.

    The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Rat
    Human
    Chinese hamster
    See all applications and species data

  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr14 of human Cdk2.

  • Positive control

    • WB: HeLa,C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours, C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours, CHO-S (Chinese hamster ovary epithelial cell) whole cell lysates. IHC: Human ovarian carcinoma, mouse and rat stomach tissues.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)

    Lane 1 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
    Lane 2 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    Western blot - Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265)
    All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)

    Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate
    Lane 3 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 4 : Untreated C2C12 (Mouse myoblasts myoblast) whole cell lysate
    Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate
    Lane 6 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa

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