Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Plasmin Inhibitor Screening Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729) is based on the ability of Plasmin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 360/450 nm. In the presence of Plasmin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Plasmin.
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Notes
Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasmin inhibitors are critical in the treatment of hyperfibrinolysis-associated blood loss and related complications.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Plasmin Assay Buffer 1 x 15ml Plasmin Dilution Buffer 1 x 1.5ml Plasmin Enzyme 1 x 15µl Plasmin Inhibitor (Aprotinin, 0.6 mM) 1 x 100µl Plasmin Substrate 1 x 200µl
Images
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Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729)
Inhibition of Plasmin activity by a Plasmin Inhibitor (Aprotinin).
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Plasmin Inhibitor Screening Assay Kit (Fluorometric) (ab204729)Plasmin activity was measured in plasma samples in the presence and absence of a Plasmin inhibitor, Aprotinin. S = Substrate, I = Inhibitor, AB = Activation Buffer containing Urokinase.
