Anti-Cdk2 antibody (ab64669)
Key features and details
- Rabbit polyclonal to Cdk2
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cdk2 antibody
See all CDK2 primary antibodies -
Description
Rabbit polyclonal to Cdk2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cdk2.
Read Abcam's proprietary immunogen policy (Peptide available as ab71917.) -
Positive control
- This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, HeLa Whole Cell - Bleomycin Treated (20U/ml), Jurkat Whole Cell, Y79 Whole Cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseCowDogChinese hamsterSyrian hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).Target
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Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 100689039 Chinese hamster
- Entrez Gene: 519217 Cow
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: O55076 Chinese hamster
- SwissProt: Q5E9Y0 Cow
see all -
Alternative names
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
Images
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Western blot - Anti-Cdk2 antibody (ab64669)
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Western blot - Anti-Cdk2 antibody (ab64669)All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab64669 has been referenced in 3 publications.
- Zheng X et al. MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts. Braz J Med Biol Res 51:e7574 (2018). PubMed: 30365725
- Duan M et al. Obovatol inhibits the growth and aggressiveness of tongue squamous cell carcinoma through regulation of the EGF-mediated JAK-STAT signaling pathway. Mol Med Rep 18:1651-1659 (2018). PubMed: 29845251
- Kim JJ et al. WSB1 overcomes oncogene-induced senescence by targeting ATM for degradation. Cell Res 27:274-293 (2017). PubMed: 27958289
Images
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Western blot - Anti-Cdk2 antibody (ab64669)
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Western blot - Anti-Cdk2 antibody (ab64669)All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
