Recombinant Human VEGFB protein (ab179970)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: ELISA, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 50 µg/ml.
Images
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SDS-PAGE - Recombinant Human VEGFB protein (ab179970)
SDS-PAGE analysis of reduced ab179970 stained overnight with Coomassie Blue. As a result of glycosylation, DTT-reduced protein migrates as 15-18 kDa and non-reduced protein migrates as 33-35 kDa.
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ELISA - Recombinant Human VEGFB protein (ab179970)Immobilized ab179970 at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1 with a linear range of 2-32 ng/mL.