Anti-StAR antibody (ab58013)
Key features and details
- Mouse monoclonal to StAR
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-StAR antibody
See all StAR primary antibodies -
Description
Mouse monoclonal to StAR -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment: EAMQKALGIL SNQEGWKKES QQDNGDKVMS KVVPDVGKVF RLEVVVDQPM ERLYEELVER MEAMGEWNPN VKEIKVLQKI GKDTFITHEL AAEAAGNLVG , corresponding to amino acids 81-180 of Human StAR
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-StAR antibody (ab58013)Anti-StAR antibody (ab58013) at 1/1000 dilution + Mouse liver lysates at 50 µg
Secondary
HRP-conjugated Goat polyclonal to mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 27,30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesLiver lysates prepared from mouse injected with StarD1 adenovirus expressing recombinant StarD1 protein.
Primary diluted in TBST + 2.5% milk and incubated with sample for 12 hours at 4°C.
This image was generated using the ascites version of the product.
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Western blot - Anti-StAR antibody (ab58013) This image is courtesy of an anonymous AbreviewAnti-StAR antibody (ab58013) at 1/1000 dilution (in TBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human THP1 cells at 50 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocking Step: 5% Milk for 4 hours at 22°C
This image was generated using the ascites version of the product.
