Androstenedione ELISA Kit (ab178609)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 20 pg/ml - 1000 pg/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Androstenedione ELISA Kit
See all Androstenedione kits -
Detection method
Colorimetric -
Precision
Inter-assay Sample n Mean SD CV% Saliva 10 -
Sample type
Saliva -
Assay type
Competitive -
Sensitivity
5 pg/ml -
Range
20 pg/ml - 1000 pg/ml -
Recovery
102.6 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in saliva.
A 96-well plate has been precoated with anti-Androstenedione antibodies. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP conjugate for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 50X Washing Solution 1 x 20ml Androstenedione HRP Conjugate 1 x 1ml Androstenedione Standard 0 - 0 pg/mL 1 x 1ml Androstenedione Standard 1 - 20 pg/mL 1 x 1ml Androstenedione Standard 2 - 100 pg/mL 1 x 1ml Androstenedione Standard 3 - 400 pg/mL 1 x 1ml Androstenedione Standard 4 - 1,000 pg/mL 1 x 1ml Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit Control A 1 vial Control B 1 vial Cover Foil 1 unit Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- 4 androstenedione
Images
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Typical Standard Curve
Representative standard curve using ab178609.
