Androstenedione ELISA Kit (ab108672)
Key features and details
- Sensitivity: 0.01 ng/ml
- Range: 0.1 ng/ml - 10 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Androstenedione ELISA Kit
See all Androstenedione kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Control Sera 60 Inter-assay Sample n Mean SD CV% Control Sera 30 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 0.01 ng/ml -
Range
0.1 ng/ml - 10 ng/ml -
Recovery
100.91 %
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Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.
A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Androstenedione Control 1 x 1ml Androstenedione Standard 0 – 0 ng/mL 1 x 1ml Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml Androstenedione-HRP Conjugate 1 x 21ml Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Alternative names
- 4 androstenedione
Images
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Typical Standard Curve
Representative Standard Curve using ab108672
