Human CG beta ELISA Kit (CGB3) (ab272194)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.62 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CG beta ELISA Kit (CGB3) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 5% -
Sample type
Urine, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.62 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 90 85% - 93% Serum 90 85% - 96% EDTA Plasma 105 96% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CG beta ELISA kit (ab272194) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CG beta protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CG beta with 2.62 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Chorionic Gonadotropin (hCG) is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated and normally produced by placenta during pregnancy. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. CG beta (CGB3) has an essential role in maternal adaption and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however, the absolute increase of β-hCG level in choriocarcinoma patients clearly differentiates it from normal pregnancy.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CG beta (CGB3) Capture Antibody 1 x 600µl 10X Human CG beta (CGB3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CG beta (CGB3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma. -
Cellular localization
Secreted -
Alternative names
- anterior pituitary glycoprotein hormones common subunit alpha
- CG alpha
- CG-ALPHA
see all -
Database links
- Entrez Gene: 1082 Human
- Entrez Gene: 1081 Human
- Omim: 118860 Human
- Omim: 118850 Human
- SwissProt: P01233 Human
- SwissProt: P01215 Human
- Unigene: 119689 Human
- Unigene: 446683 Human
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CG beta standard curve in Sample Diluent NS.The CG beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CG beta in human serum and plasma samples.The concentrations of CG beta were measured in duplicates, interpolated from the CG beta standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 1:1000 and pregnant plasma (EDTA) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CG beta concentration was determined to be 666.7 ng/mL in pregnant serum and 1,589 ng/mL in pregnant plasma (EDTA).
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Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (ab272194)To learn more about the advantages of recombinant antibodies see here.
