Anti-Cdk9 antibody [EP3118] (ab75848)
![Anti-Cdk9 antibody [EP3118] (ab75848)](https://www.abcam.com/ps/products/75/ab75848/Images/ab75848-90970-anti-cdk9-antibody-ep3118-immunohistochemistry.gif "Anti-Cdk9 antibody [EP3118] (ab75848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3118] to Cdk9
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cdk9 antibody [EP3118]
See all Cdk9 primary antibodies -
Description
Rabbit monoclonal [EP3118] to Cdk9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT-29, HeLa and 293T cell lysates. Human breast carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3118 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EP3118] (ab75848)
ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Cdk9 antibody [EP3118] (ab75848)](https://www.abcam.com/ps/products/75/ab75848/Images/ab75848-90969-anti-cdk9-antibody-ep3118-western-blot.gif)
Western blot - Anti-Cdk9 antibody [EP3118] (ab75848)All lanes : Anti-Cdk9 antibody [EP3118] (ab75848) at 1/2000 dilution
Lane 1 : HT-29 cell
lysate
Lane 2 : HeLa cell
lysate
Lane 3 : 293T cell
lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
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![Anti-Cdk9 antibody [EP3118] (ab75848)](https://www.abcam.com/ps/products/75/ab75848/Images/ab75848-3-benefits-of-recombinant-antibodies.png)
Anti-Cdk9 antibody [EP3118] (ab75848)
