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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Cell cycle Kinases/phosphatases Cdks

Anti-Cdk9 antibody (ab227211)

Anti-Cdk9 antibody (ab227211)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cdk9
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cdk9 antibody
    See all Cdk9 primary antibodies
  • Description

    Rabbit polyclonal to Cdk9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Cdk9 (internal sequence). The exact sequence is proprietary.
    Database link: P50750

  • Positive control

    • WB: HL-60 and NIH/3T3 whole cell lysates. IHC-P: Rat adrenal gland tissue; Mouse brain tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Images

  • Western blot - Anti-Cdk9 antibody (ab227211)
    Western blot - Anti-Cdk9 antibody (ab227211)
    Anti-Cdk9 antibody (ab227211) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Western blot - Anti-Cdk9 antibody (ab227211)
    Western blot - Anti-Cdk9 antibody (ab227211)
    Anti-Cdk9 antibody (ab227211) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab227211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab227211)

    Paraffin-embedded mouse brain tissue stained for Cdk9 using ab227211 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab227211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab227211)

    Paraffin-embedded rat adrenal gland tissue stained for Cdk9 using ab227211 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab227211)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab227211)

    4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cdk9 (green) using ab227211 at 1/500 dilution in ICC/IF.

    Red: Phalloidin at 1/200 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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