Rat IL-9 ELISA Kit (ab252353)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.9 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-9 ELISA Kit
See all IL-9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 6.8% -
Sample type
Serum, Cell culture media, Tissue Culture Media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.9 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 79 78% - 79% Cell culture media 98 96% - 101% Tissue Culture Media 103 99% - 107% Hep Plasma 76 75% - 77% EDTA Plasma 91 89% - 94% Cit plasma 84 83% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-9 ELISA Kit (ab252353) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cit plasma, edta plasma, hep plasma, serum, tissue culture media, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-9 with 7.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-9 Capture Antibody 1 x 600µl 10X Rat IL-9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat IL-9 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
Sequence similarities
Belongs to the IL-7/IL-9 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine P40
- Homolog of mouse T cell and mast cell growth factor 40
- HP40
see all -
Database links
- Entrez Gene: 116558 Rat
- Unigene: 92374 Rat
Images
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Other - Rat IL-9 ELISA Kit (ab252353)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat IL-9 standard curve in Sample Diluent NS.The IL-9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-9 in rat tissue culture supernatant samples.Rat spleen tissue was cultured in the presence of 10 ng/mL PMA and 10 ng/mL rat IL-1a for 4 days (PMA + IL-1a) or 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 3 days (PMA + calcium ionomycin). Spleen tissue was cultured without treatment for 2 days (unstimulated). The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: PMA + IL-1a 100%, PMA + calcium ionomycin 100%, and unstimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-9 concentration was determined to be 91.51 pg/mL in neat PMA + IL-1a stimulated spleen tissue supernatant, 126.3 pg/mL in neat PMA + calcium ionomycin stimulated spleen tissue supernatant, and 19.64 pg/mL in neat unstimulated spleen tissue supernatant.
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Sandwich ELISA - Rat IL-9 ELISA Kit (ab252353)To learn more about the advantages of recombinant antibodies see here.
