Rat IL-2 ELISA Kit (ab221834)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.9 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 4% Inter-assay Sample n Mean SD CV% Serum 5 4.2% -
Sample type
Serum, Cell culture media, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.9 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93 89% - 100% Tissue Extracts 45 43% - 48% Cell culture media 108 99% - 113% Cit plasma 86 84% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-2 ELISA Kit (ab221834) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-2 protein in serum, cell culture media, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-2 with 4.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical monomeric polypeptide. It is secreted by activated CD4+ and CD8+ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, natural killer cell (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, thereby regulating cytokine cascades that correlate with each of the Th differentiation states. Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of IL-2 correlates with reduced function of CD4+CD25+ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-2 Capture Antibody 1 x 600µl 10X Rat IL-2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Rat IL-2 Lyophilized Recombinant Protein 2 vials Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Database links
- Entrez Gene: 116562 Rat
- SwissProt: P17108 Rat
- Unigene: 9871 Rat
Images
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Other - Rat IL-2 ELISA Kit (ab221834)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat IL-2 standard curveExample of rat IL-2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Rat IL-2 standard curveExample of rat IL-2 standard curve in Sample Diluent 60BS. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples.Interpolated concentrations of native IL-2 in Concanavalin A treated rat spleen (2 days) supernatant samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2 concentration was determined to be 6401 pg/mL in Concanavalin A treated rat spleen supernatant.
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Interpolated concentrations of spiked IL-2 in rat serum and plasma samples.Interpolated concentrations of spiked IL-2 in rat serum and plasma samples. The concentrations of IL-2 were measured in duplicates, interpolated from the IL-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants.Comparison of IL-2 in unstimulated and Concanavalin A stimulated rat spleen supernatants. Rat spleen was cultured for 2 days in the presence or absence of 5 µg/mL Concanavalin A. The concentrations of IL-2 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-2 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-2 concentration was determined to be 6042 pg/mL in Concanavalin A stimulated neat rat spleen supernatant and undetectable in unstimulated rat spleen supernatant and RPMI media (not shown).
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Sandwich ELISA - Rat IL-2 ELISA Kit (ab221834)To learn more about the advantages of recombinant antibodies see here.
