Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdk9 antibody (ab39098)

Key features and details

  • Rabbit polyclonal to Cdk9
  • Suitable for: ICC/IF, WB
  • Reacts with: Human, Xenopus laevis
  • Isotype: IgG

Overview

  • Product name

    Anti-Cdk9 antibody
    See all Cdk9 primary antibodies

  • Description

    Rabbit polyclonal to Cdk9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Chicken, Cow

  • Immunogen

    Synthetic peptide corresponding to Human Cdk9 aa 150-250 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab39097)

  • Positive control

    • This antibody gives a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HEK 293 (Human embryonic kidney cell line)

Properties

Applications

Our Abpromise guarantee covers the use of ab39098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Western blot - Anti-Cdk9 antibody (ab39098)
    Western blot - Anti-Cdk9 antibody (ab39098)
    All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)
    Lane 4 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



    It is documented that Cdk9 has another isoform at 50 kDa. ab39098 appears to only detect isoform 1 at 43 kDa.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab39098)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab39098)
    ICC/IF image of ab39098 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39098, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-Cdk9 antibody (ab39098)
    Western blot - Anti-Cdk9 antibody (ab39098)
    Anti-Cdk9 antibody (ab39098) at 1/250 dilution + Xenopus laevis gastrula embryo extract (Stage12) at 0.5 µg

    Secondary
    Goat Anti-rabbit IgG Alexa Fluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa


    Exposure time: 5 minutes

    See Abreview

References (1)

Publishing research using ab39098? Please let us know so that we can cite the reference in this datasheet.

ab39098 has been referenced in 1 publication.

  • Zhang X & Tao W Long Noncoding RNA LINC00152 Facilitates the Leukemogenesis of Acute Myeloid Leukemia by Promoting CDK9 Through miR-193a. DNA Cell Biol 38:236-242 (2019). PubMed: 30707636

Images

  • Western blot - Anti-Cdk9 antibody (ab39098)
    Western blot - Anti-Cdk9 antibody (ab39098)
    All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)
    Lane 4 : HEK-293 whole cell lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.



    It is documented that Cdk9 has another isoform at 50 kDa. ab39098 appears to only detect isoform 1 at 43 kDa.
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab39098)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab39098)
    ICC/IF image of ab39098 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39098, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-Cdk9 antibody (ab39098)
    Western blot - Anti-Cdk9 antibody (ab39098)
    Anti-Cdk9 antibody (ab39098) at 1/250 dilution + Xenopus laevis gastrula embryo extract (Stage12) at 0.5 µg

    Secondary
    Goat Anti-rabbit IgG Alexa Fluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa


    Exposure time: 5 minutes

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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