Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)
![Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)](https://www.abcam.com/ps/products/256/ab256311/Images/ab256311-2-anti-girk2-antibody-epr22347-2-immunoprecipitation-brain-rat.jpg "Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22347-2] to GIRK2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free
See all GIRK2 primary antibodies -
Description
Rabbit monoclonal [EPR22347-2] to GIRK2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
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General notes
Ab256311 is the carrier-free version of ab229913. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256311 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22347-2 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)
GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: rat brain lysate (10μg) (Input).
Lane 2: ab229913 IP in rat brain lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229913).
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![Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)](https://www.abcam.com/ps/products/256/ab256311/Images/ab256311-1-anti-girk2-antibody-epr22347-2-immunoprecipitation-hippocampus-mouse.jpg)
Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Mouse hippocampus lysate (10μg) (Input).
Lane 2: ab229913 IP in mouse hippocampus lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229913).
-
![Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)](https://www.abcam.com/ps/products/256/ab256311/Images/ab256311-3-benefits-of-recombinant-antibodies.png)
Anti-GIRK2 antibody [EPR22347-2] - BSA and Azide free (ab256311)
