Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 16, 2021

Anti-GIRK2 antibody [EPR22347-2] (ab229913)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22347-2] to GIRK2
Suitable for: IP, WB
Reacts with: Mouse, Rat

Product name

Anti-GIRK2 antibody [EPR22347-2]
See all GIRK2 primary antibodies
Description

Rabbit monoclonal [EPR22347-2] to GIRK2
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

Rat

WB
Mouse

Rat

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse Rat
WB	Mouse Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22347-2
Isotype

IgG
Research areas

Western blot - Anti-GIRK2 antibody [EPR22347-2] (ab229913)

All lanes : Anti-GIRK2 antibody [EPR22347-2] (ab229913) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 48 kDa
Observed band size: 48 kDa

Exposure time: 10 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Negative control: Liver tissue (PMID: 8929423)

This antibody reacts with a undetermined protein near 90KD.
Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)

GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1: rat brain lysate (10μg) (Input).

Lane 2: ab229913 IP in rat brain lysate (Input).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 7 seconds
Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)

GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

Lane 1: Mouse hippocampus lysate (10μg) (Input).

Lane 2: ab229913 IP in mouse hippocampus lysate (Input).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 7 seconds.
Anti-GIRK2 antibody [EPR22347-2] (ab229913)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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