Anti-GIRK2 antibody [EPR22347-2] (ab229913)
![Anti-GIRK2 antibody [EPR22347-2] (ab229913)](https://www.abcam.com/ps/products/229/ab229913/Images/ab229913-1-anti-girk2-antibody-epr22347-2-western-blot-mouse-rat.jpg "Anti-GIRK2 antibody [EPR22347-2] (ab229913)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22347-2] to GIRK2
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-GIRK2 antibody [EPR22347-2]
See all GIRK2 primary antibodies -
Description
Rabbit monoclonal [EPR22347-2] to GIRK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22347-2 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GIRK2 antibody [EPR22347-2] (ab229913)All lanes : Anti-GIRK2 antibody [EPR22347-2] (ab229913) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 10 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Negative control: Liver tissue (PMID: 8929423)
This antibody reacts with a undetermined protein near 90KD.
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![Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)](https://www.abcam.com/ps/products/229/ab229913/Images/ab229913-3-anti-girk2-antibody-epr22347-2-immunoprecipitation-brain-rat.jpg)
Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: rat brain lysate (10μg) (Input).
Lane 2: ab229913 IP in rat brain lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
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![Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)](https://www.abcam.com/ps/products/229/ab229913/Images/ab229913-2-anti-girk2-antibody-epr22347-2-immunoprecipitation-hippocampus-mouse.jpg)
Immunoprecipitation - Anti-GIRK2 antibody [EPR22347-2] (ab229913)GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Mouse hippocampus lysate (10μg) (Input).
Lane 2: ab229913 IP in mouse hippocampus lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
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![Anti-GIRK2 antibody [EPR22347-2] (ab229913)](https://www.abcam.com/ps/products/229/ab229913/Images/ab229913-4-benefits-of-recombinant-antibodies.png)
Anti-GIRK2 antibody [EPR22347-2] (ab229913)
