Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)
![Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)](https://www.abcam.com/ps/products/174/ab174629/Images/ab174629-311519-anti-abcc9-antibody-n319a-14-c-terminal-western-blot.jpg "Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)")
Key features and details
- Mouse monoclonal [N319A/14] to ABCC9 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-ABCC9 antibody [N319A/14] - C-terminal -
Description
Mouse monoclonal [N319A/14] to ABCC9 - C-terminal -
Host species
Mouse -
Specificity
ab174629 does not crossreact with SUR2B. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit
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Immunogen
Recombinant fragment within Mouse ABCC9 aa 1500 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P70170 -
Positive control
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S319A-14 to N319A/14, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N319A/14 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629) at 1/1000 dilution + Rat brain membrane lysate at 10 µg
Secondary
Goat anti-mouse IgG at 1/200 dilution
Predicted band size: 174 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)](https://www.abcam.com/ps/products/174/ab174629/Images/ab174629-311520-anti-abcc9-antibody-n319a-14-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCC9 antibody [N319A/14] - C-terminal (ab174629)4% formaldehyde-fixed SK-N-BE cells labeling ABCC9 using ab174629 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).