Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)
![Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)](https://www.abcam.com/ps/products/81/ab81555/Images/ab81555-412771-anti-ubxn6ubxd1-antibody-2f8-24-western-blot-human-knockout-lysate.jpg "Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)")
Key features and details
- Mouse monoclonal [2F8-24] to UBXN6/UBXD1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-UBXN6/UBXD1 antibody [2F8-24]
See all UBXN6/UBXD1 primary antibodies -
Description
Mouse monoclonal [2F8-24] to UBXN6/UBXD1 -
Host species
Mouse -
Specificity
Only detects the full-length isoform. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human UBXN6/UBXD1.
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Positive control
- WB: HEK293T, HeLa and U20S cell lysate
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General notes
This product was previously labelled as UBXN6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 0.5% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2F8-24 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : UBXN6 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaLanes 1-3: Merged signal (red and green). Green - ab81555 observed at 50 kDa. Red - loading control ab181602 observed at 36 kDa.
ab81555 Anti-UBXN6/UBXD1 antibody [2F8-24] was shown to specifically react with UBXN6/UBXD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266689 (knockout cell lysate ab258747) was used. Wild-type and UBXN6/UBXD1 knockout samples were subjected to SDS-PAGE. ab81555 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)](https://www.abcam.com/ps/products/81/ab81555/Images/ab81555-96734-anti-ubxn6-antibody-2f8-24-western-blot.jpg)
Western blot - Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555)All lanes : Anti-UBXN6/UBXD1 antibody [2F8-24] (ab81555) at 1 µg/ml
Lane 1 : U20S cells transfected with UBXN6/UBXD1 shRNA
Lane 2 : U20S cells transfected with mismatch control shRNA
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
