Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)
![Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)](https://www.abcam.com/ps/products/269/ab269961/Images/ab269961-390500-antiusp16antibodyepr2091447westernblotrattestistissuelysate20ug.jpg "Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-USP16 antibody [EPR20914-47] - BSA and Azide free
See all USP16 primary antibodies -
Description
Rabbit monoclonal [EPR20914-47] to USP16 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat testis and liver lysates; Mouse testis lysate; MEF whole cell lysate.
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General notes
ab269961 is the carrier-free version of ab236628. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20914-47 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)All lanes : Anti-USP16 antibody [EPR20914-47] (ab236628) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate at 20 µg
Lane 2 : Rat liver tissue lysate at 20 µg
Lane 3 : Mouse testis tissue lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26323689).
Exposure time: Lanes 1-3: 3 minutes Lane 4: 48 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab236628).
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![Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)](https://www.abcam.com/ps/products/269/ab269961/Images/ab269961-2-benefits-of-recombinant-antibodies.png)
Anti-USP16 antibody [EPR20914-47] - BSA and Azide free (ab269961)