Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15228] to PRPF8/Prp8
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-PRPF8/Prp8 antibody [EPR15228]
See all PRPF8/Prp8 primary antibodies
Description

Rabbit monoclonal [EPR15228] to PRPF8/Prp8
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, HepG2, K562, 293T, mouse RAW 264.7 and rat PC12 cell lysates. Human ovarian carcinoma and rat spleen tissues.
General notes
Previously labelled as PRPF8.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15228
Isotype

IgG
Research areas

Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

All lanes : Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/5000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 274 kDa
Observed band size: 274 kDa
Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/20000 dilution + 293T cell lysate at 10 µg

Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 274 kDa
Observed band size: 274 kDa
Western blot - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

All lanes : Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347) at 1/1000 dilution

Lane 1 : mouse RAW 264.7 cell lysate
Lane 2 : rat PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 274 kDa
Observed band size: 274 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

Immunohistochemical analysis of paraffin-embedded, Human ovarian carcinoma tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling PRPF8/Prp8 with ab190347 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-PRPF8/Prp8 antibody [EPR15228] (ab190347)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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