Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15229] to PRPF8/Prp8 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free
    See all PRPF8/Prp8 primary antibodies

  • Description

    Rabbit monoclonal [EPR15229] to PRPF8/Prp8 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human gastric cancer tissue.

  • General notes

    ab239003 is the carrier-free version of ab185547 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as PRPF8.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Flow Cytometry - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1:100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Immunocytochemistry/ Immunofluorescence analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling PRPF8/Prp8 with Purified ab185547 at 1:100 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue sections labeling PRPF8/Prp8 using unpurifed ab185547 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Immunocytochemistry/ Immunofluorescence - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Flow Cytometry - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Flow Cytometry - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Flow Cytometry analysis of K562 cells labeling PRPF8/Prp8 using unpurified ab185547 at a 1/190 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling PRPF8/Prp8 with Purified ab185547 at 1:2000 dilution (0.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185547).

  • Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)
    Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PRPF8/Prp8 antibody [EPR15229] - BSA and Azide free (ab239003)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.