Anti-SFPQ antibody (ab264197)
Key features and details
- Rabbit polyclonal to SFPQ
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SFPQ antibody
See all SFPQ primary antibodies -
Description
Rabbit polyclonal to SFPQ -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SFPQ aa 225-275. The exact sequence is proprietary.
Database link: P23246 -
Positive control
- IHC-P: Human colon carcinoma tissue; WB: HeLa and NIH/3T3 whole cell lysates; IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SFPQ antibody (ab264197)All lanes : Anti-SFPQ antibody (ab264197) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µgDetected with chemilimuniscence with exposure time of 3 seconds.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
4-8% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody (ab264197)Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human colon carcinoma tissue labeling SFPQ with ab264197 at 1/200 dilution. Detection: DAB.
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Immunoprecipitation - Anti-SFPQ antibody (ab264197)SFPQ was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264197 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264197 at 1 µg/ml.
Lane 1: ab264197 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
