Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SFPQ antibody [EPR11847] (ab177149)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11847] to SFPQ
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-SFPQ antibody [EPR11847]
See all SFPQ primary antibodies
Description

Rabbit monoclonal [EPR11847] to SFPQ
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide within Human SFPQ aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P23246
Positive control
- WB: Neuro-2a, NIH/3T3, RAW 264.7, HEK-293T, A431, HeLa and Jurkat whole cell lysate (ab7899). IHC-P: Human colon, pancreas and testis tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR11847
Isotype

IgG
Research areas

Western blot - Anti-SFPQ antibody [EPR11847] (ab177149)

All lanes : Anti-SFPQ antibody [EPR11847] (ab177149) at 1/10000 dilution

Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 5 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 76 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] (ab177149)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SFPQ with ab177149 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SFPQ with ab177149 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SFPQ with ab177149 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SFPQ with ab177149 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-SFPQ antibody [EPR11847] (ab177149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com