Anti-SFPQ antibody (ab99357)
Key features and details
- Rabbit polyclonal to SFPQ
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SFPQ antibody
See all SFPQ primary antibodies -
Description
Rabbit polyclonal to SFPQ -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
A region within amino acids 657-707 of Human SFPQ (NP_005057.1).
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Positive control
- HeLa whole cell lysate. Mouse NIH3T3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab99357 was affinity purified using an epitope specific to SFPQ immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SFPQ antibody (ab99357)All lanes : Anti-SFPQ antibody (ab99357) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Exposure time: 3 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody (ab99357)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling SFPQ with ab99357 at 1/500 (0.4 µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-SFPQ antibody (ab99357)ab99357 at 1 µg/ml detecting SFPQ in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
For immunoprecipitation, ab99357 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99357
Lane 2; IP using control IgG.
