Anti-nmt55 / p54nrb antibody - N-terminal (ab227014)
Key features and details
- Rabbit polyclonal to nmt55 / p54nrb - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-nmt55 / p54nrb antibody - N-terminal
See all nmt55 / p54nrb primary antibodies -
Description
Rabbit polyclonal to nmt55 / p54nrb - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Chimpanzee -
Immunogen
Recombinant fragment within Human nmt55/ p54nrb (N terminal). The exact sequence is proprietary.
Database link: Q15233 -
Positive control
- IHC-P: Mouse brain tissue; Cal27 xenograft tissue. ICC/IF: HeLa cells. WB: HepG2 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-nmt55 / p54nrb antibody - N-terminal (ab227014) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa10% SDS PAGE gel.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for nmt55 / p54nrb (green) using ab227014 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde for 15 minutes at RT.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 dilution.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody - N-terminal (ab227014)
Paraffin-embedded mouse brain tissue stained for nmt55 / p54nrb using ab227014 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody - N-terminal (ab227014)
Paraffin-embedded Cal27 xenograft tissue stained for nmt55 / p54nrb using ab227014 at 1/500 dilution in immunohistochemical analysis.