Anti-nmt55 / p54nrb antibody (ab70335)
Key features and details
- Rabbit polyclonal to nmt55 / p54nrb
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-nmt55 / p54nrb antibody
See all nmt55 / p54nrb primary antibodies -
Description
Rabbit polyclonal to nmt55 / p54nrb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to a region between residues 350 and 400 of human nmt55/ p54nrb (NP_031389.3).
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Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-nmt55 / p54nrb antibody (ab70335)All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody (ab70335)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling nmt55 / p54nrb with ab70335 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-nmt55 / p54nrb antibody (ab70335)Detection of Human nmt55 / p54nrb by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70335 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP. -
Immunocytochemistry/ Immunofluorescence - Anti-nmt55 / p54nrb antibody (ab70335)ICC/IF image of ab70335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody (ab70335)IHC image of ab70335 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70335, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-nmt55 / p54nrb antibody (ab70335)All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.1 µg/ml
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lane 5 : C6 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 30 seconds
