Anti-Gemin 5 antibody - C-terminal (ab229640)
Key features and details
- Rabbit polyclonal to Gemin 5 - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Gemin 5 antibody - C-terminal
See all Gemin 5 primary antibodies -
Description
Rabbit polyclonal to Gemin 5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Gemin 5 (C terminal). The exact sequence is proprietary.
Database link: Q8TEQ6 -
Positive control
- ICC/IF: HeLa cells. IP: HepG2 whole cell extracts. WB: HeLa and HepG2 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody - C-terminal (ab229640)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Gemin 5 (green) using ab229640 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-Gemin 5 antibody - C-terminal (ab229640)All lanes : Anti-Gemin 5 antibody - C-terminal (ab229640) at 1/500 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 169 kDa5% SDS-PAGE
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Immunoprecipitation - Anti-Gemin 5 antibody - C-terminal (ab229640)Gemin 5 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 µg of ab229640. Western blot was performed from the immunoprecipitate using ab229640.
Lane 1: Control IgG IP in HepG2 whole cell extracts.
Lane 2: ab229640 IP in HepG2 whole cell extracts.
