Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gemin 5 antibody [EPR17841] (ab201691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17841] to Gemin 5
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Gemin 5 antibody [EPR17841]
    See all Gemin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR17841] to Gemin 5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, K562 and HEK-293 cell lysates ICC/IF: HeLa and Jurkat cells. Flow Cyt: HEK-293 cells. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17841

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Western blot - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    All lanes : Anti-Gemin 5 antibody [EPR17841] (ab201691) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
    Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 168 kDa
    Observed band size: 168 kDa


    Exposure time: 7 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Immunocytochemistry/ Immunofluorescence - Anti-Gemin 5 antibody [EPR17841] (ab201691)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Gemin 5 with ab201691 at 1/600 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab201691 at 1/600 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Flow Cytometry - Anti-Gemin 5 antibody [EPR17841] (ab201691)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling Gemin 5 with ab201691 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Immunoprecipitation - Anti-Gemin 5 antibody [EPR17841] (ab201691)

    Gemin 5 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201691 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab201691 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201691 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201691 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-Gemin 5 antibody [EPR17841] (ab201691)
    Anti-Gemin 5 antibody [EPR17841] (ab201691)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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