Anti-nmt55 / p54nrb antibody (ab50411)
Key features and details
- Goat polyclonal to nmt55 / p54nrb
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-nmt55 / p54nrb antibody
See all nmt55 / p54nrb primary antibodies -
Description
Goat polyclonal to nmt55 / p54nrb -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog, Cynomolgus monkey
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Immunogen
Synthetic peptide:
C-NRAAPGAEFAPNK
, corresponding to C terminal amino acids 455-467 of Human nmt55/ p54nrb -
Positive control
- Mouse Brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-nmt55 / p54nrb antibody (ab50411)Anti-nmt55 / p54nrb antibody (ab50411) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-nmt55 / p54nrb antibody (ab50411)All lanes : Anti-nmt55 / p54nrb antibody (ab50411) at 1/2000 dilution
Lane 1 : MEF whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey anti-goat IgG HRP
Predicted band size: 55 kDa
Observed band size: 55 kDa
This image was submitted courtesy of an Abreview, please refer to Abreview for further experimental details. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody (ab50411)ab50411 at 3.8 µg/ml staining nmt55 / p54nrb in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
