Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IBP160 antibody [EPR16942] (ab205303)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16942] to IBP160
  • Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IBP160 antibody [EPR16942]
    See all IBP160 primary antibodies

  • Description

    Rabbit monoclonal [EPR16942] to IBP160

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HepG2 and HeLa whole cell lysates; human fetal kidney lysate; HeLa nuclear and cytosolic fractions. IHC-P: Human cervical carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HEK-293 cells. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    All lanes : Anti-IBP160 antibody [EPR16942] (ab205303) at 1/10000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 171 kDa
    Observed band size: 171 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    Anti-IBP160 antibody [EPR16942] (ab205303) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 171 kDa
    Observed band size: 171 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    Western blot - Anti-IBP160 antibody [EPR16942] (ab205303)
    All lanes : Anti-IBP160 antibody [EPR16942] (ab205303) at 1/1000 dilution

    Lane 1 : HeLa cytosolic fraction
    Lane 2 : HeLa nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 171 kDa
    Observed band size: 171 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IBP160 antibody [EPR16942] (ab205303)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IBP160 antibody [EPR16942] (ab205303)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling IBP160 with ab205303 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human cervical carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-IBP160 antibody [EPR16942] (ab205303)
    Immunocytochemistry/ Immunofluorescence - Anti-IBP160 antibody [EPR16942] (ab205303)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IBP160 with ab205303 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab205303 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody  at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-IBP160 antibody [EPR16942] (ab205303)
    Flow Cytometry - Anti-IBP160 antibody [EPR16942] (ab205303)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling IBP160 with ab205303 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-IBP160 antibody [EPR16942] (ab205303)
    Immunoprecipitation - Anti-IBP160 antibody [EPR16942] (ab205303)

    IBP160 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab205303 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab205303 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: ab205303 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205303 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-IBP160 antibody [EPR16942] (ab205303)
    Anti-IBP160 antibody [EPR16942] (ab205303)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.