Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UAP56 antibody [EPR13144-62] (ab181061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13144-62] to UAP56
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-UAP56 antibody [EPR13144-62]
    See all UAP56 primary antibodies

  • Description

    Rabbit monoclonal [EPR13144-62] to UAP56

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, Jurkat and 293 cell lysate. Human infiltrating duct breast carcinoma tissue. HeLa and Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Western blot - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    All lanes : Anti-UAP56 antibody [EPR13144-62] (ab181061) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Immunohistochemical analysis of paraffin embedded Human infiltrating duct breast carcinoma tissue sections labeling UAP56 using ab181061 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Immunocytochemistry/ Immunofluorescence - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181061 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry - Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Flow Cytometry - Anti-UAP56 antibody [EPR13144-62] (ab181061)

    Flow Cytometry analysis of Jurkat cells labeling UAP56 using ab181061 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Anti-UAP56 antibody [EPR13144-62] (ab181061)
    Anti-UAP56 antibody [EPR13144-62] (ab181061)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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