Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CELF4 antibody [EPR11824] (ab171740)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11824] to CELF4
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CELF4 antibody [EPR11824]
See all CELF4 primary antibodies
Description

Rabbit monoclonal [EPR11824] to CELF4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human CELF4. The exact sequence is proprietary.
Database link: Q9BZC1
Positive control
- WI-38, A431, HeLa, NIH:OVCAR-3 and SH-SY5Y cell lysates; Human pancreas and kidney tissues; permeabilized HeLa cells.
General notes
This product was previously labelled as BRUNOL4

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11824
Isotype

IgG
Research areas

Flow Cytometry - Anti-CELF4 antibody [EPR11824] (ab171740)

Flow cytometric analysis of permeabilized Hela cells labeling CELF4 using ab171740 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF4 antibody [EPR11824] (ab171740)

Immunohistochemistry of paraffin-embedded Human kidney tissue labeling CELF4 using ab171740 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF4 antibody [EPR11824] (ab171740)

Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling CELF4 using ab171740 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CELF4 antibody [EPR11824] (ab171740)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate labeling CELF4 or (2) 1X PBS (negative control) using ab171740 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-CELF4 antibody [EPR11824] (ab171740)

All lanes : Anti-CELF4 antibody [EPR11824] (ab171740) at 1/1000 dilution

Lane 1 : WI-38 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH:OVCAR-3 cell lysate
Lane 5 : SH-SY5Y cell lysate

Lysates/proteins at 1/10 dilution per lane.

Predicted band size: 52 kDa
Anti-CELF4 antibody [EPR11824] (ab171740)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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