Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDC40 antibody [EPR12539] (ab175924)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12539] to CDC40
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CDC40 antibody [EPR12539]
    See all CDC40 primary antibodies

  • Description

    Rabbit monoclonal [EPR12539] to CDC40

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CDC40 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60508

  • Positive control

    • HeLa, K562, 293T, HUVEC cell lysates, colon tissue, transitional cell carcinoma tissue, HeLa and K562 Kcells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (ab175924)

    Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CDC40 antibody [EPR12539] (ab175924)
    Flow Cytometry - Anti-CDC40 antibody [EPR12539] (ab175924)

    Flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
    Western blot - Anti-CDC40 antibody [EPR12539] (ab175924)
    All lanes : Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 66 kDa

  • Anti-CDC40 antibody [EPR12539] (ab175924)
    Anti-CDC40 antibody [EPR12539] (ab175924)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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