Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4418] to IgG2 - BSA and Azide free
  • Suitable for: ELISA, WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IgG2 antibody [EPR4418] - BSA and Azide free
    See all IgG2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4418] to IgG2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab186920 does not cross-react with Human IgG1, IgG3 or IgG4.

  • Tested applications

    Suitable for: ELISA, WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human tonsil and thyroid gland carcinoma tissue. ICC/IF: Raji cells. Flow Cyt: Raji cells.

  • General notes

    Ab186920 is the carrier-free version of ab134050. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab186920 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4418

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Flow Cytometry - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling IgG2 with purified ab134050 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134050)
  • Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunocytochemistry/ Immunofluorescence - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling IgG2 with purified ab134050 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG2 with purified ab134050 at 1:40000 dilution (0.025 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134050)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IgG2 with ab134050 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134050).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue labeling IgG2 with ab134050 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134050).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)
    Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-IgG2 antibody [EPR4418] - BSA and Azide free (ab186920)

© 2021 Astana Biomed Group LLP. All rights reserved.