Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23237-2] to TGF beta Receptor II - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free
    See all TGF beta Receptor II primary antibodies

  • Description

    Rabbit monoclonal [EPR23237-2] to TGF beta Receptor II - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HT-1080 cell. Flow Cyt: HT-1080 cell.

  • General notes

    ab270609 is the carrier-free version of ab270440. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)
    Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HT-1080 (Human fibrosarcoma cell line) cells labelling TGF beta receptor II with ab270440 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in HT-1080 cell line. Negative control: MCF-7 (Human breast adenocarcinoma cell line). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270440).

  • Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)
    Flow Cytometry - Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)

    Flow cytometric analysis of permeabilized MCF7 (Human breast adenocarcinoma epithelial cell - Left) and  HT-1080 (Human fibrosarcoma cell - Right) cells labelling TGF beta receptor II with ab270440 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF-7 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270440).

  • Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)
    Anti-TGF beta Receptor II antibody [EPR23237-2] - BSA and Azide free (ab270609)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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