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Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [C362] to Uroplakin IIIB - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free
    See all Uroplakin IIIB primary antibodies

  • Description

    Mouse monoclonal [C362] to Uroplakin IIIB - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: Mouse bladder tissue lysate. IHC-P: Mouse bladder tissue section.

  • General notes

    ab255744 is the carrier-free version of ab237778. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255744 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
    Western blot - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
    All lanes : Anti-Uroplakin IIIB antibody [C362] (ab237778) at 1/1000 dilution

    Lane 1 : Mouse bladder tissue lysate
    Lane 2 : Mouse lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab237778, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: mouse lung (PMID: 12446744).
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12446744).

    Exposure time: 26 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)

    This data was developed using ab237778 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse bladder tissue labeling Uroplakin IIIB with ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining in mouse urothelium.The section was incubated with ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)

    This data was developed using ab237778 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Uroplakin IIIB with ab237778 at 1/5000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Negative control: No staining in mouse lung.The section was incubated with ab237778 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)
    Anti-Uroplakin IIIB antibody [C362] - BSA and Azide free (ab255744)

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